CGSC Strain#: 3033
Strain Designation: AB3033      Source of Strain: P. Howard-Flanders
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1 or lacZ4, tsx-33, glnX44(AS), galK2(Oc), λ^-, Rac-0, recA13, rpsL31(strR) or rpsL8, kdgK51, xylA5, mtl-1, argE3(Oc), thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• recA13-- Amino acid substitution Leu 51 Phe
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• = strep-resistant
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References:

• Howard-Flanders, P, L Theriot, JB Stedeford 1969. Some properties of excision-defective recombination-deficient mutants of Escherichia coli K-12. J. Bacteriol. 97(3):1134-41.