CGSC Strain#: 3078
Strain Designation: GY854      Source of Strain: R. Devoret
Other Designations: AB3078
Other Designations: NH4395
Sex: F' Episome/Plasmid:
   Plasmid Markers/Mutations: lacZ545(Am)
Episome/Plasmid: F42
   Plasmid Markers/Mutations: lac
Chromosomal Markers: thr-1, leuB6(Am), fhuA21, codA1, lacY1, glnV44(AS), λ^-, uvrB501, pyrF32, thyA6, malA19, thiE1, deoC1, λ^R
Strain Comments:

• Ref. to ancestral strain #KMBL90.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• thyA6-- : Even with Luria or other complete media, supplement with thymine.
• λ^R-- Lambda resistant, locus unknown.
• glnV44(AS) was formerly called supE44
• glnV44(AS) was formerly called su⁺_II
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Am = amber(UAG) mutation

References:

• van de Putte, P, CA van Sluis, J van Dillewijn, A Rorsch 1965. The location of genes controlling radiation sensitivity in Escherichia coli. Mutat. Res. 2(2):97-110.