CGSC Strain#: 4257
Strain Designation: KL736      Source of Strain: M.C. Cappello
Sex: F' Episome/Plasmid: F122
   Plasmid Markers/Mutations:
Chromosomal Markers: leuB6(Am), fhuA2, lacY1, tsx-1, glnV44(AS), gal-6, λ^-, hisG1(Fs), recA1, thyA23, argG6, rpsL104, malT1(λ^R), xyl-7, mtlA2, metB1
Strain Comments:

• This strain must be maintained in medium containing his, leu, met and lacking arg, thy.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• thyA23-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• glnV44(AS) was formerly called supE44
• glnV44(AS) was formerly called su⁺_II
• xyl-7 was formerly called xylA7
• = strep-resistant
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Fs = Frameshift mutation
• LamR = Lambda-resistant

References:

• Low, KB 1972. Escherichia coli K-12 F-prime factors, old and new. 36(4):587-607.