CGSC Strain#: 4543
Strain Designation: AT915     
Source of Strain: A.L. Taylor
Sex: Hfr
Point of Origin#: 45
Map Position: 65.00
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: dapD12::Mu,
λ-,
e14-,
relA1,
thiE1Strain Comments: - This strain will grow on rich media without the addition of diaminopimelic acid. On minimal medium it responds best to lysine. The strain may carry a temperature-sensitive mutation.
- dapD12::Mu-- mutation is due to the insertion of phage Mu in the dapD locus.
- dapD12::Mu-- Strains with this mutation require 100ug/ml diaminopimelic acid even when grown on LB
- e14--- : Loss of the e14 element makes the strain McrA-.
- thiE1 was formerly called thi-1
References: - Bukhari, A.I., A.L. Taylor 1971. Genetic analysis of diaminopimelic acid- and lysine-requiring mutants of Escherichia coli. J.Bacteriol. 105:844-854