CGSC Strain#: 4616
Strain Designation: S26R1e, LAM-      Source of Strain: A. Garen
Other Designations: K90
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22, phoA4(Am), λ^-, ompF627(T2R), serU132(AS), fadL701(T2R), relA1, pitA10, spoT1, rrnB-2, mcrB1, creC510
Strain Comments:

• phoA4(Am)-- is Garen's phoAS26 . It is suppressed by leuX151, glnV33(=supE33) and serU132(=supD32).
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• serU132(AS) was formerly called supD32
• serU132(AS) was formerly called Su1⁺
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Const = constitutive
• T2R = T2 Resistant

References:

• Garen, A., S. Garen, R. Wilhelm 1965. Suppressor genes for nonsense mutations. I. the Su-1, Su-2, and Su-3 genes of Escherichia coli. J.Mol.Biol. 14:167-187
• Hoffman, E.P., R. Wilhelm 1970. Genetic mapping and dominance of the amber suppressor, Su1 (supD) in Escherichia coli K-12. J.Bacteriol. 103:32-36