CGSC Strain#: 4680
Strain Designation: C90     
Source of Strain: A. Garen
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10,
fhuA22,
ompF627(T2R)
,
fadL701(T2R)
,
relA1,
pitA10,
spoT1,
pst-9,
rrnB-2,
mcrB1,
creC510Strain Comments: - This strain is constitutive for alkaline phosphatase.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- pst-9-- pstA or B
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- pst-9 was formerly called phoT9
- Const = constitutive
- T2R = T2 Resistant
References: - Garen, A., S. Garen 1963. Genetic evidence on the nature of the repressor for alkaline phosphatase in E. coli. J.Mol.Biol. 6:433-438
- Willsky, G.R., M. Malamy 1976. Control of the synthesis of alkaline phosphatase and the phosphate-binding protein in Escherichia coli. J.Bacteriol. 127:595-609