CGSC Strain#: 4866
Strain Designation: JC411-5-2     
Source of Strain: J. Gerhart
Sex: F'
Episome/Plasmid: F20
Plasmid Markers/Mutations: Chromosomal Markers: leuB6(Am)
,
fhuA2,
glnV44(AS)
,
gal-6,
λ-,
pyrF92,
hisG1(Fs)
,
argG6,
rpsL104,
malT1(λ
R)
,
xyl-7,
mtlA2,
metB1Strain Comments: - This strain should be maintained on medium containing leu and his and lacking arg and met.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnV44(AS) was formerly called supE44
- glnV44(AS) was formerly called su+II
- xyl-7 was formerly called xylA7
- = strep-resistant
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Fs = Frameshift mutation
- LamR = Lambda-resistant
References: - Gerhart, JC, H Holoubek 1967. The purification of aspartate transcarbamylase of Escherichia coli and separation of its protein subunits. J. Biol. Chem. 242(12):2886-92.