CGSC Strain#: 12121
Strain Designation: BW23423     
Source of Strain: B.L. Wanner
Sex: F-
Chromosomal Markers: Δ(araD-araB)767,
araBp-NFLAG
-phoR801,
phnCp-lacZ,
Δ(phoB-phoR)580,
Δ(ackA-hisP)862,
Δ(creB-creD)153,
[phnB]Strain Comments: - araBp-NFLAG-phoR801-- A transcriptional fusion of the araB promoter to the C-terminal cytoplasmic domain of phoR (residues M95 to S384) in single copy at the araCBAD locus
- phnCp-lacZ-- A transcriptional fusion of the phnC promoter to the lacZ gene in single copy at the lacZ locus
- [phnB]-- functional phn locus from E. coli B
- araBp-NFLAG-phoR801 was formerly called araBADAH37::ParaB-NFLAG-`phoRAH41
- Δ(ackA-hisP)862 was formerly called Δ(pta-hisP)201
- Δ(araD-araB)767 was formerly called araBADAH37
- phnCp-lacZ was formerly called PphnC-lacZWJ19
References: - Haldimann, A, MK Prahalad, SL Fisher, SK Kim, CT Walsh, BL Wanner 1996. Altered recognition mutants of the response regulator PhoB: a new genetic strategy for studying protein-protein interactions. Proc. Natl. Acad. Sci. U.S.A. 93(25):14361-6.