CGSC Strain#: 12312
Strain Designation: DH5α/pLAU43      Source of Strain: D.J. Sherratt
Other Designations: DH5alpha/pLAU43
Other Designations: pLAU43
Sex: F- Episome/Plasmid: pLAU43
   Plasmid Markers/Mutations: lacZα, ori_pMB1, bla , kan
   Plasmid Comment: pFX92 with two cassettes of 120 tandem copies of the tetO operator separated by a kan gene. The insert was cloned using the NheI and XbaI sites. pFX92 is a derivative of pUC18 with the following MCS (-EcoRI-BamHI-XhoI-NheI-SmaI-XbaI-SalI-BglII-HindIII-).
Chromosomal Markers: Δ(argF-lac)169, φ80dlacZ58(M15), ΔphoA8, glnX44(AS), λ^-, deoR481, rfbC1, gyrA96(NalR), recA1, endA1, thiE1, hsdR17
Strain Comments:

• 1) This strain is to be used for non-commercial research use only. It is not intended for any animal or human therapeutic or diagnostic use.
• 2) The depositors of this strain and the CGSC assume no responsibility or liability for any special, incidental, indirect or consequential loss or damage whatsoever.
• 3) No other warranty is made, whether expressed or implied, including any warranty of merchantability or fitness for a particular purpose.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• deoR481-- A selectable phenotype of deoR mutants includes the ability to grow preferentially on minimal medium with inosine as the sole carbon source.
• gyrA96(NalR)-- resistant to 200ug/ml nalidixic acid
• gyrA96(NalR)-- GAC to AAC at codon 87
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• gyrA96(NalR) was formerly called
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• = good miniprep dsDNA quality
• AS = amber(UAG) suppressor
• NalR = Nalidix.acidResist.

References:

• Lau, IF, SR Filipe, B Søballe, OA Økstad, FX Barre, DJ Sherratt 2003. Spatial and temporal organization of replicating Escherichia coli chromosomes. Mol. Microbiol. 49(3):731-43.