CGSC Strain#: 12313
Strain Designation: AB1157/pLAU44      Source of Strain: D.J. Sherratt
Other Designations: pLAU44
Sex: F- Episome/Plasmid: pLAU44
   Plasmid Markers/Mutations:
Chromosomal Markers: thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnV44(AS), galK2(Oc), λ^-, Rac-0, hisG4(Oc), rfbC1, mgl-51, rpoS396(Am), rpsL31(strR), kdgK51, xylA5, mtl-1, argE3(Oc), thiE1
Strain Comments:

• 1) This strain is to be used for non-commercial research use only. It is not intended for any animal or human therapeutic or diagnostic use.
• 2) The depositors of this strain and the CGSC assume no responsibility or liability for any special, incidental, indirect or consequential loss or damage whatsoever.
• 3) No other warranty is made, whether expressed or implied, including any warranty of merchantability or fitness for a particular purpose.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnV44(AS) was formerly called supE44
• glnV44(AS) was formerly called su⁺_II
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Lau, IF, SR Filipe, B Søballe, OA Økstad, FX Barre, DJ Sherratt 2003. Spatial and temporal organization of replicating Escherichia coli chromosomes. Mol. Microbiol. 49(3):731-43.