CGSC Strain#: 5746
Strain Designation: JM96     
Source of Strain: M.C. Jones-Mortimer
Sex: F-
Chromosomal Markers: thr-1,
leuB6(Am)
,
fhuA2,
lacY1,
glnV44(AS)
,
gal-6,
λ-,
trp-1,
hisG1(Fs)
,
rfbC1,
cysH56,
galP63,
Δ(gltB-gltF)500,
malT1(λ
R)
,
xyl-7,
mtlA2,
ΔargH1,
rplL9(L?)
,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- Δ(gltB-gltF)500-- :Glt- phenotype in gltB mutant requires presence of a gdhA mutation as well. 0.2% aspartate is a better N-source than glutamate because of uptake(Pahel et al).
- Δ(gltB-gltF)500 was formerly called gltB31
- Δ(gltB-gltF)500 was formerly called aspB21
- Δ(gltB-gltF)500 was formerly called aspB24
- glnV44(AS) was formerly called supE44
- glnV44(AS) was formerly called su+II
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Fs = Frameshift mutation
- L? = assignment uncertain
- LamR = Lambda-resistant
References: - Jones-Mortimer, MC 1968. Positive control of sulphate reduction in Escherichia coli. Isolation, characterization and mapping oc cysteineless mutants of E. coli K12. Biochem. J. 110(3):589-95.
- Jones-Mortimer, MC 1973. Mapping of structural genes for the enzymes of cysteine biosynthesis in Escherichia coli K12 and Salmonella typhimurium LT2. Heredity 31(2):213-21.