CGSC Strain#: 5768
Strain Designation: MI226a      Source of Strain: M. Iaccarino
Sex: F- Chromosomal Markers: araBAD-9, fhuA1, lacZ13(Oc), tsx-3, λ^-, trpE3(Oc), hisG4(Oc), rpsL8 or rplL9(L?), malT1(λ^R), ilvG603(Act), ilvG605(Am), thiE1
Strain Comments:

• This strain is IlvG^- due to the ilvG605 mutation.
• lacZ13(Oc)-- is lacZ₂ of Jacob, originating in Paris strain 3.320. It is an ochre, polar mutation.
• ilvG603(Act)-- is an insertion mutation which activates the ilvG gene.
• ilvG605(Am)-- is an amber mutation.
• thiE1 was formerly called thi-1
• Act = activates the Gene
• Am = amber(UAG) mutation
• LamR = Lambda-resistant
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Favre, R, A Wiater, S Puppo, M Iaccarino 1976. Expression of a valine-resistant acetolactate synthase activity mediated by the ilv O and ilv G genes of Escherichia coli K-12. Mol. Gen. Genet. 143(3):243-52.
• Lawther, R.P., D.H. Calhoun, J.E. Gray, C.W. Adams, C.A. Hauser, G.W. Hatfield 1982. DNA sequence fine-structure analysis of ilvG (IlvG+) mutations of Escherichia coli K-12. J.Bacteriol. 149:294-298