CGSC Strain#: 5768
Strain Designation: MI226a     
Source of Strain: M. Iaccarino
Sex: F-
Chromosomal Markers: araBAD-9,
fhuA1,
lacZ13(Oc)
,
tsx-3,
λ-,
trpE3(Oc)
,
hisG4(Oc)
,
rpsL8 or rplL9(L?)
,
malT1(λ
R)
,
ilvG603(Act)
,
ilvG605(Am)
,
thiE1Strain Comments: - This strain is IlvG- due to the ilvG605 mutation.
- lacZ13(Oc)-- is lacZ2 of Jacob, originating in Paris strain 3.320. It is an ochre, polar mutation.
- ilvG603(Act)-- is an insertion mutation which activates the ilvG gene.
- ilvG605(Am)-- is an amber mutation.
- thiE1 was formerly called thi-1
- Act = activates the Gene
- Am = amber(UAG) mutation
- LamR = Lambda-resistant
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Favre, R, A Wiater, S Puppo, M Iaccarino 1976. Expression of a valine-resistant acetolactate synthase activity mediated by the ilv O and ilv G genes of Escherichia coli K-12. Mol. Gen. Genet. 143(3):243-52.
- Lawther, R.P., D.H. Calhoun, J.E. Gray, C.W. Adams, C.A. Hauser, G.W. Hatfield 1982. DNA sequence fine-structure analysis of ilvG (IlvG+) mutations of Escherichia coli K-12. J.Bacteriol. 149:294-298