CGSC Strain#: 5917
Strain Designation: N728      Source of Strain: D. Apirion
Sex: Hfr    Point of Origin#: 12   Map Position: 68.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: fhuA2::IS2, lacY1 or lacZ4, tsx-1, glnX44(AS), gal-6, λ^-, rimK728, purF1, rpsE2296(SpcR), xyl-7, mtlA2, metB1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• rimK728-- The multiple antibiotic resistance phenotype may be due to multiple mutations including rimK and another mutation mapping at ~74.1
• purF1-- is a very stable mutation and may be an intragenic deletion.
• rpsE2296(SpcR)-- is Apirion's spc-296. It was erroneously listed as spc-54 in the paper cited.
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rimK728 was formerly called nek
• xyl-7 was formerly called xylA7
• ::IS2 = Insertion of IS2
• AS = amber(UAG) suppressor
• SpcR = spectinomycin resistant

References:

• Brown, M.E., D. Apirion 1974. Mapping a cluster of ribosomal genes in Escherichia coli. Mol.Gen.Genet. 133:317-327