CGSC Strain#: 12350
Strain Designation: DH5&alpha/pC4-19      Source of Strain: Quan
Other Designations: DH5alpha/pC4-19
Other Designations: pC4-19
Sex: F- Chromosomal Markers: Δ(argF-lac)169, φ80dlacZ58(M15), ΔphoA8, glnV44(AS), λ^-, deoR481, rfbC1, gyrA96(NalR), recA1, endA1, thiE1, hsdR17
Strain Comments:

• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• deoR481-- A selectable phenotype of deoR mutants includes the ability to grow preferentially on minimal medium with inosine as the sole carbon source.
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• glnV44(AS) was formerly called supE44
• glnV44(AS) was formerly called su⁺_II
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• = good miniprep dsDNA quality
• AS = amber(UAG) suppressor
• NalR = Nalidix.acidResist.

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