CGSC Strain#: 12351
Strain Designation: DE418     
Source of Strain: F. Stahl
Other Designations: DE418/pLM2
Sex: F-
Episome/Plasmid: pLM2
Plasmid Markers/Mutations: kan,
ampR(Am)
,
tet(Am)
,
kan Plasmid Comment: Broad host-range plasmid, derivative of RP1, but with amber mutations in bla and tet
Chromosomal Markers: λ-,
thyA36,
IN(rrnD-rrnE)1,
rph-1,
rpoB331(rifR)
,
hsdR19Strain Comments: - It is believed that this plasmid is in a W3110 background, but that has not been positively established. It is documented to be a non-suppressing host so it should be sensitive to ampicillin and tetracycline.
- thyA36-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- rifR = rifamycin resistance
- = kanamycin resistant
- Am = amber(UAG) mutation
References: - Mindich, L., C.J. Cohen, M. Weisburd 1976. Isolation of nonsense suppressor mutants of Pseudomonas. J.Bacteriol. 126:177-182
- Seed, B 1983. Purification of genomic sequences from bacteriophage libraries by recombination and selection in vivo. Nucleic Acids Res. 11(8):2427-45.