MC1009/pSL118      Source of Strain:
C.L. SquiresOther Designations:
pSL118 Plasmid Markers/Mutations: cat
, ori[pMB1] Plasmid Comment:
Derived from pKK232-8 Chromosomal Markers: Δ(araA-leu)7697
, mcrB1Strain Comments:
- Δ(araA-leu)7697-- Gerdes et al. show this deletion to cover from 63.4 kb to 89 kb, from polB through fruR.
- Δ(araA-leu)7697-- Shown by Durfee et al. to be a deletion of the first 295 codons of hepA to the first 82 codons of fruR.
- [araD139]B/r-- The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B.
- Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
- Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
- Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
- galK16-- An IS2 insertion resulting in the truncation of the protein at codon 56
- galE15(GalS)-- G to A transition resulting in an S122F amino acid substitution
- galE15(GalS)-- is Brenner's gal-U38, isolated by UV mutagenesis from his strain CA1.
- e14--- : Loss of the e14 element makes the strain McrA-.
- recA56-- Amino acid substitution Arg 60 Cys
- srlD300::Tn10-- : The insertion was determined by sequencing to be within srlD (F.W.Pons, pers.commun., Oct.2000)
- rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- Δ(araA-leu)7697 was formerly called Δ(hepA-fruR)7697
- Δ(codB-lacI)3 was formerly called Δ(lac)3
- Δ(codB-lacI)3 was formerly called Δlac74
- Δ(codB-lacI)3 was formerly called DE(lac)6
- Δ(codB-lacI)3 was formerly called X74
- Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
- srlD300::Tn10 was formerly called srlC300::Tn10
- = tetracycline resistant
- GalS = UDPGal accum.toxic if GalKRP+
- strR = streptomycin resist.
- ApR = ampicillin resistant
- Li, SC, CL Squires, C Squires 1984. Antitermination of E. coli rRNA transcription is caused by a control region segment containing lambda nut-like sequences. Cell 38(3):851-60.