CGSC Strain#: 7010
Strain Designation: RL25      Source of Strain: R. Lathe
Other Designations: RC269
Sex: Hfr    Point of Origin#:    Map Position: Chromosomal Markers: fhuA67, lpxD200(ts), relA1, spoT1, metB1
Strain Comments:

• lpxD200(ts)-- This allele has three amino acid substitutions compared to WT, Q165R, G228D, and G252S
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• lpxD200(ts) was formerly called firA200
• ts = Temperature Sensitive

References:

• Lathe, R., H. Buc, J.-P. Lecocq, E.K.F. Bautz 1980. Prokaryotic histone-like protein interacting with RNA polymerase. Proc.Natl.Acad.Sci.USA 77:3548-3552