CGSC Strain#: 12503
Strain Designation: S17-1 λpir/pLFX      Source of Strain: T. Romeo
Other Designations: S17-1 Lambda pir/pLFX
Other Designations: pLFX
Sex: F- Episome/Plasmid: pLFX
   Plasmid Markers/Mutations:
Chromosomal Markers: RP4-2(Km::Tn7,Tc::Mu-1), pro-82, LAMpir, recA1, endA1, thiE1, hsdR17, creC510
Strain Comments:

• RP4-2(Km::Tn7,Tc::Mu-1)-- integrated plasmid is Kan and Tet sensitive, due to the insertions in Tc and Km, and the Tn7 results in TRIM/aminopterin resistance and low level strR.
• RP4-2(Km::Tn7,Tc::Mu-1)-- The RP4-2 also has an ISR1 insertion element within the Tn1³⁵ transposon that inactivates the adjacent ampicillin resistance gene.
• pro-82-- is in proA or proB.
• LAMpir-- Allows the replication of plasmids with an R6Kγ origin of replication
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• thiE1 was formerly called thi-1
• = good miniprep dsDNA quality
• Const = constitutive

References:

• Edwards, AN, LM Patterson-Fortin, CA Vakulskas, JW Mercante, K Potrykus, D Vinella, MI Camacho, JA Fields, SA Thompson, D Georgellis, M Cashel, P Babitzke, T Romeo 2011. Circuitry linking the Csr and stringent response global regulatory systems. Mol. Microbiol. 80(6):1561-80.