CGSC Strain#: 6480
Strain Designation: JS10      Source of Strain: Sturgeon & Ingram
Sex: F- Chromosomal Markers: araC14, leuB6(Am), secA206(aziR), fhuA23, proA180, lacZ36, tsx-67, purE42, glnX44(AS), λ^-, trpE38, lysA23, rpsL109(strR), ftsX2000(Cs), xylA5, mtl-1, metE70, thiE1
Strain Comments:

• This strain is cold-sensitive. Grow at 39 degrees C.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• purE42-- :Strains with this marker are derivatives of Χ148 and may carry malA38LAMR and a suppressor of the Mal^- phenotype, sup-78,near his.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• aziR = azide resistance
• Cs = cold-sensitive
• strR = streptomycin resist.

References:

• Sturgeon, JA, LO Ingram 1978. Low-temperature conditional cell division mutants of Escherichia coli. J. Bacteriol. 133(1):256-64.
• Gill, D.R., G.F. Hatfull, G.P.C. Salmond 1986. A new cell division operon in Escherichia coli. Mol.Gen.Genet. 205:134-145