CGSC Strain#: 6363
Strain Designation: RE216      Source of Strain: R.C. Essenberg
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Episome/Plasmid: pLC35-4
   Plasmid Markers/Mutations: thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), λ^-, mtl-1, thiE1, galT22, recA56, xylA5, ΔtrpE63, uhpT , uhpB , uhpC , uhpA , ilvB
   Plasmid Comment: In RE216; host strain RE213. R. Essenberg strains. Hybridizes with Kohara clones 567-569.
Chromosomal Markers: fhuA22, ompF627(T2R), uhp-40, recA56, srlD300::Tn10, relA1, pyrE41, spoT1, tnaA6, metB1, gltSo14
Strain Comments:

• recA56-- Amino acid substitution Arg 60 Cys
• srlD300::Tn10-- : The insertion was determined by sequencing to be within srlD (F.W.Pons, pers.commun., Oct.2000)
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• gltSo14-- is Halpern's gltC7.
• srlD300::Tn10 was formerly called srlC300::Tn10
• = tetracycline resistant
• T2R = T2 Resistant
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References:

• Smith, G.R. . Personal Communication. Personal Communication
• Goldenbaum, P.E., K.S. Farmer 1980. uhp-directed, glucose 6-phosphate membrane receptor in Escherichia coli. J.Bacteriol. 142:347-349
• Neidhardt, F.C., V. Vaughn, T.A. Phillips, P.L. Bloch 1983. Gene-protein index of Escherichia coli K-12. Microbiol.Rev. 47:231-284