CGSC Strain#: 5291
Strain Designation: BB20     
Source of Strain: Cronan & Bell
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
ΔphoA8,
fadL701(T2R)
,
relA1,
glpR2(glpc),
glpD3,
pitA10,
gpsA20,
spoT1,
rrnB-2,
mcrB1,
creC510Strain Comments: - glpD3-- Loss of G-3-P dehydrogenase (aerobic) results in inability of strains to grow on glycerol or glycerol-3-P as sole carbon source.
- glpD3-- GlpD loss also results in stasis with gluconeogenic C sources, e.g.,succinate. glpA or glpD mutants should be grown on min.salts+1% glycerol-free casein hydrolysate+1% glucose.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- Const = constitutive
- glpc = constitutive expr.glp regulon
- T2R = T2 Resistant
References: - Cronan, J.E., R.M. Bell 1974. Mutants of Escherichia coli defective in membrane phospholipid synthesis: mapping of the structural gene for L-glycerol 3-phosphate dehydrogenase. J.Bacteriol. 118:598-605