CGSC Strain#: 4255
Strain Designation: KL732      Source of Strain: M.C. Cappello
Other Designations: EWH287
Other Designations: KLF17/KL132
Sex: F' Episome/Plasmid: F117
   Plasmid Markers/Mutations:
Chromosomal Markers: thr-1, leuB6(Am), fhuA2::IS2, pro-27, lacY1, glnX44(AS), gal-6, λ^-, hisG1, recA1, thyA25, malT1(λ^R), xyl-7, mtlA2, rplL9(L?), thiE1, pyrB31
Strain Comments:

• This strain must be grown on medium containing thi, his, leu, pro, thr, thy and lacking ura.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• thyA25-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• ::IS2 = Insertion of IS2
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• L? = assignment uncertain
• LamR = Lambda-resistant

References:

• Low, KB 1972. Escherichia coli K-12 F-prime factors, old and new. 36(4):587-607.