CGSC Strain#: 6421
Strain Designation: SK2255      Source of Strain: S.R. Kushner
Sex: F- Chromosomal Markers: zbe-279::Tn10, thyA6, rpsL120(strR), deoC1
Strain Comments:

• zbe-279::Tn10-- cotransduces 66% with leuS and 80% with rna.
• thyA6-- : Even with Luria or other complete media, supplement with thymine.
• thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• = tetracycline resistant
• strR = streptomycin resist.

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