CGSC Strain#: 1019
Strain Designation: AB1019     
Source of Strain: T. Ramakrishnan & Adelberg
Sex: F'
Episome/Plasmid: F14-6
Plasmid Markers/Mutations: ilvO-266 Plasmid Comment: carries {\it ilvE266} mutation.
Chromosomal Markers: fhuA2::IS2,
lacY1 or lacZ4,
tsx-4,
glnX44(AS)
,
gal-6,
λ-,
hisG1,
rpsL8 or rplL9(L?)
or rpsL17,
malT1(λ
R)
,
xyl-7,
mtlA2,
metB1,
ΔargH1,
thiE1Strain Comments: - This strain is valine-resistant but is very unstable and segregates out valine-sensitive clones in nutrient broth.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- = strep-resistant
- ::IS2 = Insertion of IS2
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
References: - Ramakrishnan, T., E.A. Adelberg 1965. Regulatory mechanisms in the biosynthesis of isoleucine and valine. II. Identification of two operator genes. J.Bacteriol. 89:654-660