CGSC Strain#: 3587
Strain Designation: AB3587      Source of Strain: B.B. Magee
Sex: F' Chromosomal Markers: araC14, lacY1, tsx-57, glnX44(AS), glnS1(ts), galK2(Oc), metC56, xyl-7 or xylA5, thiE1, tfr-5
Strain Comments:

• This strain stops growth immediately at 42 degees C.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glnS1(ts)-- is a temperature-sensitive mutation. Growth stops immediately at 42 degrees C. This allele reverts and the strain should be cloned and tested before use.
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• ts = Temperature Sensitive

References:

• Korner, A, BB Magee, B Liska, KB Low, EA Adelberg, D Soll 1974. Isolation and partial characterization of a temperature-sensitive Escherichia coli mutant with altered glutaminyl-transfer ribonucleic acid synthetase. J. Bacteriol. 120(1):154-8.