AB3587      Source of Strain:
F' Chromosomal Markers: araC14
, xyl-7 or xylA5
, tfr-5Strain Comments:
- This strain stops growth immediately at 42 degees C.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- glnS1(ts)-- is a temperature-sensitive mutation. Growth stops immediately at 42 degrees C. This allele reverts and the strain should be cloned and tested before use.
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- ts = Temperature Sensitive
- Korner, A, BB Magee, B Liska, KB Low, EA Adelberg, D Soll 1974. Isolation and partial characterization of a temperature-sensitive Escherichia coli mutant with altered glutaminyl-transfer ribonucleic acid synthetase. J. Bacteriol. 120(1):154-8.