CGSC Strain#: 4180
Strain Designation: EM139     
Source of Strain: E. Murgola
Sex: F-
Chromosomal Markers: araC14,
lacY1,
tsx-57,
glnX44(AS)
,
galK2(Oc)
,
rpsL20(strR)
,
xylA5,
mtl-1,
thiE1,
tfr-5,
ilv-257,
Ssl-139Strain Comments: - This strain must be grown in the presence of 200ug/ml streptomycin; otherwise, faster-growing revertants of the streptomycin-suppressible lethal mutation Ssl-139 will be selected.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- Ssl-139-- streptomycin-suppressible lethal mutation.
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Murgola, EJ, EA Adelberg 1970. Streptomycin-suppressible lethal mutations in Escherichia coli. J. Bacteriol. 103(1):20-6.