CGSC Strain#: 5118
Strain Designation: JE3513     
Source of Strain: E. Ohtsubo
Sex: F'
Episome/Plasmid: F8-33
Plasmid Markers/Mutations: traA-,
tolA ,
tolB ,
gal Chromosomal Markers: thr-1,
leuB6(Am)
,
fhuA57,
lac-52,
galK2(Oc)
or galT23,
LAMind-,
rpsL197(strR)
,
malT1(λ
R)
,
xylA5,
mtl-1,
fim-2,
fla-5302,
pho-80,
tsx-76 or tsx-459Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- fim-2 was formerly called pil-2
- Am = amber(UAG) mutation
- LamR = Lambda-resistant
- strR = streptomycin resist.
References: - Ohtsubo, E, MT Hsu 1978. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F100, F152, and F8 and mapping of the Escherichia coli chromosomal region fep-supE-gal-attlambda-uvrB. J. Bacteriol. 134(3):778-94.