CGSC Strain#: 5618
Strain Designation: WA2548      Source of Strain: W. Arber
Other Designations: AC5618
Sex: F- Chromosomal Markers: leuB6(Am), fhuA21, lacY1 or Δ(cod-lacI)6, glnX44(AS), λ^-, metB1, thiE1, hsdS12
Strain Comments:

• This is a K-12 derivative which has received the hsd- deoB region from E. coli 15. This strain is r^-_A, m^-_A.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor

References:

• Arber, W, D Wauters-Willems 1970. Host specificity of DNA produced by Escherichia coli. XII. The two restriction and modification systems of strain 15T-. Mol. Gen. Genet. 108(3):203-17.