CGSC Strain#: 5728
Strain Designation: Lin 298      Source of Strain: E.C.C. Lin
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22, ΔphoA8, fadL701(T2R), relA1, glpR2(glp^c), pitA10, spoT1, glpK22(fbR), rrnB-2, mcrB1, creC510, mgr-8
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• glpK22(fbR)-- This allele is both Ki (insensitive to inhibition by fructose bisphosphate) and Kr (resistant to inhibition by IIAglu of the PTS) . Sequenced allele: G304S.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive
• fbR = feedback resistant
• glp^c = constitutive expr.glp regulon
• T2R = T2 Resistant

References:

• Freedberg, W.B., W.S. Kistler, E.C.C. Lin 1971. Lethal synthesis of methylglyoxal by Escherichia coli during unregulated glycerol metabolism. J.Bacteriol. 108:137-144