CGSC Strain#: 14201
Strain Designation: WTZ17003     
Source of Strain: J.E. Wertz
Sex: F-
Chromosomal Markers: Δ(araD-araB)567,
ΔlacZ4787(::rrnB-3)
,
λ-,
Δ(malQ-malP)2096(::kan),
rph-1,
Δ(rhaD-rhaB)568,
hsdR514Strain Comments: - Strain constructed by the CGSC from parent strain BW25113
- Δ(araD-araB)567-- This deletion extends from ~25 bp upstream of the araB start codon to ~8 bp into the beginning of the araD gene
- ΔlacZ4787(::rrnB-3)-- : 4 tandem copies of the rrnB transcriptional terminator inserted by gene replacement into the region extending from near the SacII site near the N-terminus of lacZ through the promoter.
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- Δ(araD-araB)567 was formerly called Δ araBADAH33
- Δ(rhaD-rhaB)568 was formerly called Δ rhaBADLD78
- ΔlacZ4787(::rrnB-3) was formerly called ::rrnB-4
- ΔlacZ4787(::rrnB-3) was formerly called Δ lacZWJ16
- = kanamycin resistant
- ::rrnB-3 = 4 copies of rrnB inserted
- del-ins = deletion-insertion
References: