ASH1      Source of Strain:
Hfr Point of Origin#:
129 Map Position:
8.60 Direction of Marker Transfer:
Clockwise Chromosomal Markers: Sfa
, metB1Strain Comments:
- ASH1 transfers the chromosome predominantly from PO 129, but does give some transfer from PO 130.
- thyA117-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- strR = streptomycin resist.
- Buxton, R.S., I.B. Holland 1973. Genetic studies of tolerance to colicin E2 in Escherichia coli K12. I. Re-location and dominance relationships of cet mutations. Mol.Gen.Genet. 127:69-88