W3110trpA33      Source of Strain:
F- Chromosomal Markers: λ-
, rph-1Strain Comments:
- trpA33-- has two consecutive nucleotide substitutions (R.Milkman) and has not been known to revert (C.Yanofsky via R.Milkman).
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)