CGSC Strain#: 5752
Strain Designation: JM277     
Source of Strain: M.C. Jones-Mortimer
Sex: F'
Episome/Plasmid: F595-1
Plasmid Markers/Mutations: cysC92,
lac ,
serA ,
purE ,
lysA Plasmid Comment: This is translocated F, TOR13 ({\it serA, purE, lac, lysA, cys}).
Chromosomal Markers: leuB6(Am)
,
fhuA2::IS2,
lacY1,
tsx-1,
glnX44(AS)
,
gal-6,
hisG1,
recA1,
thyA23,
argG6,
rpsL104,
malT1(λ
R)
,
xyl-7,
mtlA2,
metB1Strain Comments: - F595 and derivatives carry translocation involving serA, purE, lac, lysA, cysC.
- The F-prime is the same as F595, but carries cysC92 and begins at the PO.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
- recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
- thyA23-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- xyl-7 was formerly called xylA7
- = strep-resistant
- ::IS2 = Insertion of IS2
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
References: