CGSC Strain#: 5752
Strain Designation: JM277      Source of Strain: M.C. Jones-Mortimer
Sex: F' Episome/Plasmid: F595-1
   Plasmid Markers/Mutations: cysC92, serA , purE , lysA , lac
   Plasmid Comment: This is translocated F, TOR13 ({\it serA, purE, lac, lysA, cys}).
Chromosomal Markers: leuB6(Am), fhuA2, lacY1, tsx-1, glnV44(AS), gal-6, hisG1(Fs), recA1, thyA23, argG6, rpsL104, malT1(λ^R), xyl-7, mtlA2, metB1
Strain Comments:

• F595 and derivatives carry translocation involving serA, purE, lac, lysA, cysC.
• The F-prime is the same as F595, but carries cysC92 and begins at the PO.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• thyA23-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• glnV44(AS) was formerly called supE44
• glnV44(AS) was formerly called su⁺_II
• xyl-7 was formerly called xylA7
• = strep-resistant
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Fs = Frameshift mutation
• LamR = Lambda-resistant

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