EM138      Source of Strain:
F- Chromosomal Markers: lacY1
, tfr-5Strain Comments:
- Streptomycin-suppressible lethal. Probably T4S - see AB1621.
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- ilv-275-- The ilv-275 mutation renders this strain conditionally streptomycon-dependent.
- galK2(Oc) was formerly called gal2
- glnV44(AS) was formerly called supE44
- glnV44(AS) was formerly called su+II
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
- Murgola, EJ, EA Adelberg 1970. Streptomycin-suppressible lethal mutations in Escherichia coli. J. Bacteriol. 103(1):20-6.