CGSC Strain#: 862
Strain Designation: D2 & JC182      Source of Strain: A.J. Clark
Other Designations: CGSC#4213
Other Designations: AB862 & AB863
Sex: Hfr    Point of Origin#:    Map Position: Chromosomal Markers: glnV44(AS), e14-, purF1, relA1, thiE1
Strain Comments:

• Double Hfr PO1 & PO12.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• purF1-- is a very stable mutation and may be an intragenic deletion.
• glnV44(AS) was formerly called supE44
• glnV44(AS) was formerly called su⁺_II
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor

References:

• Clark, A.J. 1963. Genetic analysis of a "double male" strain of Escherichia coli K-12. Genetics 48:105-120