CGSC Strain#: 2599
Strain Designation: S26R1D      Source of Strain: A. Garen
Sex: Hfr    Point of Origin#:    Map Position: Chromosomal Markers: fhuA22, phoA4(Am), glnV33(AS), fadL701(T2R), relA1, pitA10, rrnB-2, mcrB1, creC510
Strain Comments:

• supE33 = Garen's su2⁺.
• phoA4(Am)-- is Garen's phoAS26 . It is suppressed by leuX151, glnV33(=supE33) and serU132(=supD32).
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• glnV33(AS) was formerly called supE33
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Const = constitutive
• T2R = T2 Resistant

References:

• Eggertsson, G., E.A. Adelberg 1965. Map positions and specificities of suppressor mutations in Escherichia coli K-12. Genetics 52:319-340
• Garen, A., S. Garen, R. Wilhelm 1965. Suppressor genes for nonsense mutations. I. the Su-1, Su-2, and Su-3 genes of Escherichia coli. J.Mol.Biol. 14:167-187