CGSC Strain#: 4873
Strain Designation: DF2000      Source of Strain: D. Fraenkel
Sex: Hfr    Point of Origin#:    Map Position: Chromosomal Markers: garB10, fhuA22, ompF627(T2R), zwf-2, fadL701(T2R), relA1, pitA10, spoT1, rrnB-2, pgi-2, mcrB1, creC510
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• pgi-2-- : pgi mutants utilize glucose by the hexose monoP shunt; strains with this allele grew on glucose at 1/3 parental rate. Slow growth on Gal,Mal,Lac;normal on Fru and gluconate.
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive
• T2R = T2 Resistant

References:

• Peyru, G, DG Fraenkel 1968. Genetic mapping of loci for glucose-6-phosphate dehydrogenase, gluconate-6-phosphate dehydrogenase, and gluconate-6-phosphate dehydrase in Escherichia coli. J. Bacteriol. 95(4):1272-8.
• Fraenkel, DG 1968. Selection of Escherichia coli mutants lacking glucose-6-phosphate dehydrogenase or gluconate-6-phosphate dehydrogenase. J. Bacteriol. 95(4):1267-71.
• Fraenkel, D., S. Banerjee 1972. Deletion mapping of zwf, the gene for a constitutive enzyme, glucose 6-phosphate dehydrogenase in Escherichia coli. Genetics 71:481-489