G11h5      Source of Strain:
Hfr Point of Origin#:
2A Map Position:
12.20 Direction of Marker Transfer:
Counterclockwise Chromosomal Markers: relA1
, ampCp-1Strain Comments:
- "Unknown mutation" confers resistance to 50 mg/ml ampicillin; not yet mapped.
- ampCp-1-- is a promoter mutation; AmpC+ strains carrying this allele produce about 10X the amount of beta lactamase found in wild-type strains. In absence of other amp alleles, use 10-15 ug/ml Amp.
- ampCp-1 was formerly called ampA1
- Boman, HG, KG Eriksson-Grennberg, S Normark, E Matsson 1968. Resistance of Escherichia coli to penicillins. IV. Genetic study of mutants resistant to D,L-ampicillin concentrations o 100 mu-g-ml. Genet. Res. 12(2):169-85.