CGSC Strain#: 5774
Strain Designation: KL16, lac5      Source of Strain: D. Elseviers
Other Designations: DEV1
Sex: Hfr    Point of Origin#: 45   Map Position: 65.00   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: lacZ105(Am), e14-, relA1, spoT1, thiE1
Strain Comments:

• lacZ105(Am)-- is a UAG mutation.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation

References:

• Low, K.B. 1968. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc.Natl.Acad.Sci.USA 60:160-167
• Low, K.B. 1973. Rapid mapping of conditional and auxotrophic mutants of Escherichia coli K-12. J.Bacteriol. 113:798-812