CGSC Strain#: 6714
Strain Designation: GR300      Source of Strain: I.R. Booth
Sex: Hfr    Point of Origin#:    Map Position: Chromosomal Markers: fhuA22, lacY1, ompF627(T2R), relA1, zhe-923::Tn10, spoT1, metB1, thiE1, gltSo14
Strain Comments:

• zhe-923::Tn10-- cotransduces 71% with rpsL, 79% with rpsE, and 63% with kefB
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• gltSo14-- is Halpern's gltC7.
• thiE1 was formerly called thi-1
• = tetracycline resistant
• T2R = T2 Resistant

References:

• Rowland, G.C., P.M. Giffard, I.R. Booth 1984. Genetic studies of the phs locus of Escherichia coli, a mutation causing pleiotropic lesions in metabolism and pH homeostasis. FEBS Lett. 173:295-300