CGSC Strain#: 7121
Strain Designation: NI512      Source of Strain: H. Nakayama
Sex: F- Chromosomal Markers: leuB6(Am), fhuA2, lacY1, thiL49, glnV44(AS), gal-6, λ^-, thiK48, trp-31, cpxB11, hisG1(Fs), rfbC1, thiD45, galP63, argG6, rpsL104, malT1(λ^R), xyl-7, mtl-21, metB1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• thiL49-- - Note: thiL thiD double mutant does not respond to thiamin supplementation. It requires 0.1uM TPP for growth in Luria broth.
• thiK48-- - Note: a thiK thiD double is unable to grow in the presence of thiamin. Grows in Luria + 0.1 uM thiamin monophosphate.
• cpxB11-- was formerly called cpxB1.
• glnV44(AS) was formerly called supE44
• glnV44(AS) was formerly called su⁺_II
• rfbC1 was formerly called rfbD1
• xyl-7 was formerly called xylA7
• = strep-resistant
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Fs = Frameshift mutation
• LamR = Lambda-resistant

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