CGSC Strain#: 7109
Strain Designation: IQ86      Source of Strain: K. Shiba
Sex: F- Chromosomal Markers: [araD139]_B/r, Δ(argF-lac)169, λ^-, flhD5301, Δ(fruK-yeiR)725(fruA25), relA1, rpsE0(SpcR), zhe-33::Tn10, rpsL150(strR), rbsR22, Δ(fimB-fimE)632(::IS1), deoC1
Strain Comments:

• [araD139]_B/r-- The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• flhD5301-- flagellar defect shown in Komeda and Iino
• Δ(fruK-yeiR)725(fruA25)-- : this fruA25 mutation deletes 29 codons of fruK, fruB and fruBKAp promoter to orf yeiR Peters et al. J.Bact.185:2017.
• zhe-33::Tn10-- between rpsE and rpsL
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• Δ(fruK-yeiR)725(fruA25) was formerly called fruA25
• = tetracycline resistant
• ::IS1 = insertion of IS1
• fruA25 = former designation
• SpcR = spectinomycin resistant
• strR = streptomycin resist.

References:

• Shiba, K., K. Ito, T. Yura 1984. Mutation that suppresses the protein export defect of the secY mutation and causes cold-sensitive growth of Escherichia coli. J.Bacteriol. 160:696-701
• Shiba, K., K. Ito, T. Yura, D.P. Cerretti 1984. A defined mutation in the protein export gene within the spc ribosomal protein operon of Escherichia coli: isolation and characterization of a new temperature-sensitive secY mutant. EMBO J. 3:631-635