CGSC Strain#: 7141
Strain Designation: MM54      Source of Strain: J.R. Beckwith
Sex: F- Chromosomal Markers: [araD139]_B/r, leu-59::Tn10, secA51(ts), Δ(argF-lac)169, phoR9999, λ^-, flhD5301, Δ(fruK-yeiR)725(fruA25), relA1, rpsL150(strR), rbsR22, Δ(fimB-fimE)632(::IS1)
Strain Comments:

• [araD139]_B/r-- The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• phoR9999-- This is probably allele phoR82.
• flhD5301-- flagellar defect shown in Komeda and Iino
• Δ(fruK-yeiR)725(fruA25)-- : this fruA25 mutation deletes 29 codons of fruK, fruB and fruBKAp promoter to orf yeiR Peters et al. J.Bact.185:2017.
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• Δ(fruK-yeiR)725(fruA25) was formerly called fruA25
• = tetracycline resistant
• ::IS1 = insertion of IS1
• fruA25 = former designation
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Oliver, D.B., J.R. Beckwith 1981. E. coli mutant pleiotropically defective in the export of secreted proteins. Cell 25:765-772