CGSC Strain#: 7167
Strain Designation: W3110K     
Source of Strain: A. Ishihama
Other Designations: W3110 Kohara
Sex: F-
Chromosomal Markers: λ-,
IN(rrnD-rrnE)1,
rph-1Strain Comments: - IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
References: - Bachmann, BJ 1972. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol.Rev. 36(4):525-57.
- Hill, C.W., B.W. Harnish 1981. Inversions between ribosomal RNA genes of Escherichia coli. Proc.Natl.Acad.Sci.USA 78:7069-7072
- Jensen, K.F. 1993. The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. J.Bacteriol. 175:3401-3407
- Bachmann, B.J. 1996. Derivation and genotypes of some mutant derivatives of Escherichia coli K-12. In: Neidhardt et al., eds. ASM:2460-2488