CGSC Strain#: 7273
Strain Designation: UTH4771     
Source of Strain: T.S. Matney
Sex: F-
Chromosomal Markers: araC14,
lacY1,
tsx-57,
glnX44(AS)
,
galK2(Oc)
,
Rac-0,
hisH4771,
rfbC1,
mgl-51,
nadB9999,
rpsL20(strR)
,
xylA5,
mtl-1,
thiE1,
tfr-5Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Goldschmidt, E.P., M.S. Cater, T.S. Matney, M.A. Butler, A. Greene 1970. Genetic analysis of the histidine operon in Escherichia coli K12. Genetics 66:219-229
- Garrick-Silversmith, L., P.E. Hartman 1970. Histidine-requiring mutants of Escherichia coli K12. Genetics 66:231-244