CAG18420      Source of Strain: Sex:
F- Chromosomal Markers: lacZ118
, rph-1Strain Comments:
- lacZ118(Oc)-- is the lacZ allele originating in Paris strain Hfr 3000 U118. It is an ochre mutation.
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- = kanamycin resistant
- Oc = ochre(UAA) mutation
- Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of