CGSC Strain#: 7405
Strain Designation: CAG12183     
Source of Strain: C.A. Gross
Sex: F-
Chromosomal Markers: λ-,
gyrA-0(NalR)
,
zfa-3145::Tn10kan,
rph-1Strain Comments: - The gyrA allele was added to the genotype following feedback reports and subsequent testing that indicated that the strain is NalR.
- zfa-3145::Tn10kan-- is derived from zfa-723::Tn10, placed by PCR analysis in orf yfaL, nucleotide 2,341,772 (50.478 min.).
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- zfa-3145::Tn10kan was formerly called zei-3143::Tn10kan
- = kanamycin resistant
- NalR = Nalidix.acidResist.
References: - Singer, M., T.A. Baker, G. Schnitzler, S.M. Deischel, M. Goel, W.F. Dove, K.J. Jaacks, A.D. Grossman, J.. Erickson, C.A. Gross 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of
- Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.