CGSC Strain#: 7319
Strain Designation: DF52      Source of Strain:
Other Designations: GN2
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: lacI22, dctB3, λ^-, glk-1, ptsI2, relA1, spoT1, thiE1
Strain Comments:

• This strain was briefly called GN2
• ptsI2-- results in strain phenotype of no growth on mannitol or glucose, good growth on glycerol plus cAMP.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• lacI22 was formerly called lac_i3^-
• lacI22 was formerly called lacI3
• thiE1 was formerly called thi-1

References:

• FRAENKEL, DG, F FALCOZ-KELLY, BL HORECKER 1964. THE UTILIZATION OF GLUCOSE 6-PHOSPHATE BY GLUCOKINASELESS AND WILD-TYPE STRAINS OF ESCHERICHIA COLI. Proc. Natl. Acad. Sci. U.S.A. 52:1207-13.
• Tanaka, S, DG Fraenkel, EC Lin 1967. The enzymatic lesion of strain MM-6, a pleiotropic carbohydrate-negative mutant of Escherichia coli. Biochem. Biophys. Res. Commun. 27(1):63-7.
• Lo, T.C.Y., M.K. Rayman, B.D. Sanwal 1972. Transport of succinate in Escherichia coli.I. Biochemical and Genetic studies of transport in whole cells. J.Biol.Chem. 247:6323-307