CGSC Strain#: 6220
Strain Designation: KL286     
Source of Strain: M.C. Cappello
Sex: F-
Chromosomal Markers: garB10,
fhuA22,
phoA4(Am)
,
galK35,
serS14(ts)
,
serC13,
ompF627(T2R)
,
trpC45,
his-68,
fadL701(T2R)
,
relA1,
rpsL118(strR)
,
malT1(λ
R)
,
pitA10,
xyl-7,
mtlA2,
spoT1,
rrnB-2,
mcrB1,
creC510Strain Comments: - phoA4(Am)-- is Garen's phoAS26 . It is suppressed by leuX151, glnV33(=supE33) and serU132(=supD32).
- galK35-- is Brenner's gal-U99 from Hfr 3000 U99 and has been called galK99
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- xyl-7 was formerly called xylA7
- Am = amber(UAG) mutation
- Const = constitutive
- LamR = Lambda-resistant
- strR = streptomycin resist.
- T2R = T2 Resistant
- ts = Temperature Sensitive
References: - Clarke, S.J., K.B. Low, W. Konigsberg 1973. Close linkage of the genes serC (for phosphohydroxy pyruvate transaminase) and serS (for seryl-transfer ribonucleic acid synthetase) in Escherichia coli K-12. J.Bacteriol. 113:1091-1095