CGSC Strain#: 6946
Strain Designation: CSR603/pDR1996     
Source of Strain: A. Sancar
Sex: F-
Episome/Plasmid: pDR1996
Plasmid Markers/Mutations: bla(ApR)
,
uvrA+,
ssb+,
Tc(tetR)
,
uvrA ,
ssb ,
Tc ,
bla Plasmid Comment: This is a pBR322 derivative carrying uvrA and ssb (tetR from pSC101 and ampR from Tn3.) pDR1453(Sancar and Rupp 1980) obtained by sbis0001@flinders.edu.au from local Flinders Univ. source.
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
Δ(gpt-proA)62,
lacY1,
tsx-33,
glnX44(AS)
,
phr-1,
galK2(Oc)
,
λ-,
Rac-0,
gyrA98(NalR)
,
recA1,
rpsL31(strR)
,
kdgK51,
xylA5,
mtl-1,
argE3(Oc)
,
thiE1,
uvrA6Strain Comments: - pDR1996 is a pBR322 derivative and is TetR, AmpR, and carries uvrA, ssb genes.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- NalR = Nalidix.acidResist.
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
- ApR = ampicillin resistant
- tetR = tetracycline resistant
References: - Sancar, A., K.R. Williams, J.W. Chase, W.D. Rupp 1981. Sequences of the ssb gene and protein. Proc.Natl.Acad.Sci.USA 78:4274-4278